The study aimed to assess the students' knowledge and attitudes related to physiological changes of elderly people in El-Azhar University at Assuit city, Egypt. Design: A descriptive research design was utilized. Setting: This study conducted in two faculties (Education and Shariaa and low) at El-azahr University. The total sample size was 363 female students who were selected by random sample from two faculties in El-Azhar University at Assuit City. Three tools were used, tool 1: Students' socio-demographic characteristic and education background questionnaire, tool II: facts on aging quiz 2 (FAQZ) and tool III: attitude toward old people scale (ATOP). Results: 44.6% of students participants had fair knowledge, 70.2% of students participants had negative attitude, also there was a significant positive correlation between knowledge score and attitude score (r =0.114 & p = 0.029), and there was a significant positive correlation between their ages and knowledge score (r = 0.134& p = 0.011). Conclusion Majority of students had a fair score of knowledge related to physiological changes which occur to the elderly people however; the majority of them had a negative attitude toward elderly people. Recommendation: an educational program to raise the level of knowledge and attitude related to physiological changes of elderly people is needed.