Background: Incident report is an important tool which helps health care provider to manage and document their medical and administrative information. Aims: Designing, implementing training program to improve Nurses' knowledge and practice about incident report at Assuit Rajhy- Liver Hospital. Research design: A quasi-experimental design with pre, post and follow up test were utilized. Study subject: included all nurses (n=30) at Assuit Rajhy- Liver Hospital. Study tool: a tool was applied regarding incident report for (pre, post and follow up phase of the training program) about incident report. Results: There was marked improvement about nurses' knowledge and practice immediately after program implementation and retained through follow up, with highly statistical significant differences through the program phases. Conclusion: Study subject had an improvement of knowledge and practice through follow up phase. Recommendation: Maintain and apply incident report structured form developed within the present study as permanent data source at Assuit Rajhy- Liver Hospital and for University Hospitals.