Background: Worldwide, cervical cancer is the third most common type of cancer in women, it is claims the life of almost half a million women worldwide each year. Aim: To assess the effect of educational program on nurse's awareness about cervical cancer. Subject and method: A quiz experimental study design was used in this study, the sample included all nurses working in the Assiut Women's Health Hospital, they were 66 nurse. Pre and post test done to evaluate nurse's awareness about cervical cancer based on evaluation of nurses knowledge and attitude about cervical cancer .Results: The majority of the sample lived in rural area and had a diploma degree in nursing. The mean age of nurses were 29.3±9.6year, near to three-quarter of nurses had adequate knowledge about cervical cancer and more than two-thirds of them had positive attitude toward Pap smear as a screening test after implementation of the educational program, also it was observed a statistical significant relation for nurse's knowledge in pre and post test. Conclusion: There was an improvement in nurses' level of knowledge and their attitude become positive toward cervical cancer screening. Recommendations: Provide educational program about cervical cancer and its preventive measurements for nurse in all areas that provide maternity services.