Radiation is a threat to health in work place and in general environment. Workers in radiation therapy department are exposed to a variety of occupational hazards. Health care and safety measures compliance may decrease the health sequences of hazards occurrence. The study aimed to assess knowledge and practice of health care team about radiation hazards and it‟s safety measures. A descriptive design was used in this study. It was conducted at the Main Assuit University hospital and South Egypt Cancer Institute. A convenient sample (300) from heath team workers from the previous setting. Two tools was used (1) Self administrative and interview questionnaire. (2) Health team observation check list. Results: There were significant statistical differences in relation between health team knowledge and their performance. The study concluded that health team had poor knowledge about radiation hazards and most of them had satisfactory practice about safety measures. It recommend that hospitals must be provide radiation departments with adequate personal protective equipment to reduce exposure of health system from radiation hazards and establish plans for periodical safety training courses and educational programs for health team members in radiation setting to help in improving their practice and update their knowledge.