Background: The clinical learning environment is an interactive network of forces within the clinical setting that influences learning outcomes. It includes everything that surrounds students and affects their professional development in the clinical setting. Aim: was to determine internship nursing students' opinions of factors affecting their clinical learning environment in Assiut University Hospitals. Study design: A descriptive design was used. Setting: Assiut University Hospitals- Assiut Governorate – Egypt. Subjects: The study sample consisted of 200 nursing internship students. A self –administered questionnaire to gather data about personal characteristics and clinical learning environment. Results: Displayed that the high mean scores and standard deviation of the internship nursing students opinions involved instructor, learning objective, and clinical environment factors respectively. In addition, there were negative correlations in scores of internship nursing students' opinions of their clinical learning environment (learning objectives, instructor, how to deal with students in clinical settings & clinical environment) and their residence with statistically significant differences respectively except for supervision and evaluation factor. Conclusions: The most influential factors affecting the students in the area of clinical practice are the instructor, learning objectives, and clinical environment. Recommendations: Developing “Clinical Practice Guide" based on the job description for internship nursing students training areas.