Introduction: Nurses are amongst the employees whose lives are fully affected by the quality of work life (QWL) as a consequence of dynamic changes in work environment. The aim was to investigate the relationship among nurses' quality of work life, organizational culture and turnover intention at Assiut University Hospital. Design: A correlational descriptive design was used. Setting: four Intensive Care Units classified as follows: Causality Intensive Care Unit, General & Postoperative ICUs, and Coronary Care Unit (CCU).Subjects: Included all nurses working in aforementioned settings with a total number of 120 nurses. Four tools were used to collect data namely: socio-demographic data sheet, quality of nurses' work life, organizational culture questionnaire, and turnover intention questionnaire. Results: illustrated that nurses' quality of work life scores was highly significant in relation to the department, marital status, organizational culture score, and turnover intention score. Conclusion: This study contributed to understanding the relationships between quality of nurses work life and organizational culture that will lead to turnover intention among the study sample of hospital nurses and found a positive correlation between quality of nurses work life and organizational culture with highly statistically significant relation and between quality of nurses work life and turnover intention . While, there was a negative correlation between turnover intention and organizational culture. Recommendations: Workshops on the quality of work life skills for nurses and nurse managers should be done periodically. In order to lessen intent to turnover for the nursing profession, staff nurses are encouraged to participate in nursing committees, assume leadership roles, and become decision-makers.