Background: Values are essential in order to maintain the Elderly Patients' Rights. Aim of the study: to assess knowledge and attitudes of nurses regarding elderly patients' Bill of Rights and Code of Ethics at Assiut University Hospital. Subject and methods: 400 nurses participate and descriptive cross-sectional study design was used.. Total coverage technique was used. Data collection started from 1 July – 31 December 2013. Tools: an interviewing questionnaire used for data collection, it contained three parts: assess personal data, assess nurses' knowledge toward code of ethics; and elderly patients' rights, assess nursing attitudes toward code of ethics and elderly patients' rights. Results: 33.8% of the studied nurses had experience in nursing work 5-10 years. The studied nurses had satisfactory knowledge toward code of ethics and elderly patients' rights', (65% and59.2%) respectively. Regarding to gender factor; females represent significant high score of knowledge toward code of ethics than males.Conclusion: It was concluded that education level and gender can affect the knowledge level of the nurses. Recommendations: involving the professional values and elderly patients' rights in nursing educational curriculum, and implement an educational program for updating nurses' knowledge .