Background: The atmosphere in poultry houses, particularly where ventilation is limited, can adversely affect human health. Aim of study: Assess knowledge, attitude and practice of participants at poultry farms regarding occupational health hazards Methods: cross sectional study was used in this study concluded 400 participants from Assuit district One tool used; Interview questionnaire developed to collect data. It consist of five parts first part to assess socio-demographic characteristics, second part to assess medical history, third part to assess workers' knowledge, fourth part to assess practice of workers and fifth part is likert rating scale to assess workers' attitude. Results: Shows that 53.8% were 20- 40 years, 40.2 % had secondary school, 71.7% had poor knowledge about occupational health hazards at poultry farms, and 34.8 % had positive attitude towards occupational health hazards at poultry farms. Conclusion: There was poor knowledge among poultry workers in Assuit distract while more than one third had positive attitude towards their work. Recommendations:. Workers must wear appropriate personal protective equipment such as gloves, mask and aprons.