Background: Evidence-based clinical guidelines provide nurses with a method to use critically appraised. But, there are several barriers to their implementation which can be grouped as barriers related to individual nurse characteristics, organizational characteristics, and the nature of research information. The aim of the study: was to identify hindering factors for critical care nurses' compliance with evidence based guidelines for prevention of secondary brain injury. Materials & Method: Two tools were developed to collect data. “Evidence based guidelines for prevention of secondary brain injury observational checklist tool" and “Factors hindering nurses' compliance with Evidenced based guidelines for prevention of secondary brain injury questionnaire tool" to determine factors hinder nurses' compliance with evidenced based guidelines for prevention of secondary brain injury. Results: The study revealed that the overall average compliance with seizure control recommendations was the highest (91.9%) followed by maintaining adequate nutrition and preventing DVT recommendations which were more than two thirds among secondary brain injury prevention recommendations. Moreover, the organizational barriers were the most reported barriers by the majority of the CCNs (84%). Conclusion: In conclusion, the barriers to studied nurses' compliance are ranked and categorized as organizational barriers, followed by nature of research information barriers, then personal barriers.