The effect of concentrations of four chemical compounds: Furedan (0.5 mg to 4.0 mg/l). Seven (0.5 mg to 4.0 mg/l), Glyphosate (0.2 mg to 6.4 mg/l) and Stroby (1.5 mg to 12 mg/l) on in vitro growth and development of strawberry (Fragaria x ananasa Duch.) cv. “Festival" shoot tips was examined in order to optimize shoot regeneration and growth efficiencies. Shoot tip explants were obtained from in vitro-grown plantlets and Murashige and Skoog original medium was used as basal medium. The results showed that Furedan at 0.5 mg/l was optimal for all parameters measured compared with other concentrations tested. Seven at the concentration of 1.0 mg/l was better than other concentrations tested for shoot formation. The lowest concentration of Seven tested inhibited shoot elongation over the control. Leave number and root number and length were, however, largely unresponsive to all levels of Seven tested. In response to inclusion of Glyphosate in the culture medium, shoot and leaf formation were better with 0.8 mg/l than with other concentrations tested. Shoot elongation was inhibited whereas root formation and elongation were largely unaffected by all Glyphosate concentrations tested.The shoot tip explants responded best to relatively high concentrations of Stroby and the largest number of shoots was obtained on the medium containing 6.0 mg Stroby/l. On the other hand, all Stroby concentrations tested resulted in significant decrease in values of all other parameters measured relative to the control. Although it is now difficult to give an explanation for the effects of these chemicals, the results of this study demonstrate that relatively low concentrations of these chemicals have significant influence on growth and development of in vitro cultured shoot tips of the strawberry“Festival" cultivar which indicate that all chemical tested, in this study, have varying degrees of growth regulators-like actions.