Listeria monocytogenes from Listeria genus, which as saprophytes, but causes systemic infections as pathogenic bacteria. Listeria monocytogenes is widely spread in environment, caused infection of farm animals during fields grazing leading to contamination by wildlife, or manure. There was a close relation between improperly farm environment originated from contaminated crops and listeriosis. It causes animals infection with is not associated with clinical symptoms then the animal is flaking Listeria monocytogenes in feces. Listeria species' identification is a serious to gain the ecological Listeria virulence influence. The samples understudy consisted of animal stool, mattress, ration and water (n = 30) were for each. All samples were exposed to bacterial check. The results obtained that Listeria species was isolated with a general occurrence of 33.3%. The stools had 17.5%, mattress 7.5%, ration 5.0%, and water 3.3%. Meanwhile the occurrence of Listeria monocytogenes was overall 20.0% included (10.0%, 5.0%, 2.5%, and 2.5 %) as the same aforementioned samples. The PCR-technique was applied for detection of Listeria monocytogenes virulence genes using primers groups. Those genes were hlyA (hemolysin A gene), inlA (internalin A), prfA. The results obtained the present of all virulence genes. Listeria monocytogenes susceptibility to antimicrobial agents usually used for farms treatment was determined for animal stools Listeria monocytogenes isolates. The results showed that the generally sensitive rate was 70.0%. The100.0% was sensitive to Ampicillin, Amoxicillin, Erythromycin, and Tetracycline followed by Ofloxacin 75.0%. But the 50.0% was sensitive to Penicillin G, Cephalexin, and Gentamycin, finally Lincomycin was resistant. It was concluded that the Listeria monocytogenes were isolated from understudy samples as noticeable degrees. Also, virulence genes were presented in the isolated Listeria monocytogenes. That powerful proved the attendance of Listeria monocytogenes trendy a hidden contamination in animal farm. So, that could affect the animal health and animal productions. It was recommended that the undertake to follow the "Farm Health Conditions" and follow-up to decrease the microbial contamination. As well fix the animal vaccinations to maintain the animal health and livestock.