Punching is one of the most important phenomena to be considered during the design of reinforced concrete flat slabs. This research investigates, experimentally, the behavior of interior slab-column connections consisted of lightweight concrete and special shear reinforcement. The experimental work included testing of five flat slabs with dimensions of 1100 x 1100 mm and thickness of 120 mm. The studied variables were the concrete type, the presence of shear reinforcement, and the column dimensions. The concrete type varies from normal weight concrete to lightweight concrete, while the column dimensions vary from 150 x 150 mm to 250 x 250 mm. The five specimens were loaded at the interior column with a single concentrated load until failure. The test results showed that using of lightweight concrete decreased the performance of slab behavior compared with the normal weight concrete and reduced the shear punching capacity. Also, The presence of shear reinforcement improved punching shear but without a remarkable percentage, while changing the column dimensions had a remarkable effect on the punching shear capacity.