Increased range of stripe rust Puccinia striiformis tritici [Pst] virulence on wheat Triticum aestivum L. in Egypt at last decades has required assemblage of a broad genetic base of resistance. The objective of this study to identify inheritance of Yr17 and Yr27 resistance to wheat stripe rust. Eight crosses between Yr17, Yr27 and each of Giza 163, Gemmiza 3, Sids-6 and Sids-9, were performed. Results indicated that the four parents exhibited high susceptible reaction against stripe rust at seedling and adult stage. Breeding against stripe rust at seedling and adult stages proved that plant segregation indicated that F₁ plants of the eight testers having Yr17 and Yr27, were resistant and exhibited low stripe rust reaction (0-1) at seedling stage and low stripe rust severity ranged between 0 and 5R at adult stage. The result of F₂ plants reaction exhibited wide range of stripe rust severity ranged between 0 and 60S but the direction was in the side resistance and this confirmed the results of F₁. This result confirmed the presence of resistant gene in the segregations of the resulted crosses and verified that a single dominant pair gene controls stripe rust resistance at adult stages.