The laboratory and field experiments were carried out to estimate the cytotoxic effects of two synthetic and two bio-fungicides on parents, F1 and F2 faba bean plants. Data obtained from the direct treatment repeated experiments showed that both synthetic inorganic and bio-fungicides have lethal effects on all seeds so the recovery treatments were applied to study their effects on mitotic behaviour of Vicia faba parent and their F1 and F2 plants. Data showed that mitotic index (MI) values of almost all plants (seeds) treated with the three different concentrations of Dithane and Rizolex fungicides at different exposuring times were significantly lower than those of control plants. Seeds treated with 4 gm/L Dithane at 12 h exhibited the lowest value of MI (4.87%) while that value of seeds treated with Rizolex (3g/L at 24 h) was the lowest one at all (3.65%). In general, most treatments with two bio-fungicides exhibited significantly lower MI values than that of control. The MI of the treatments of Blight Stop (1.5% at 24h) and Clean Root (10% at 24h) were the lowest values 7.68% and 6.95% respectively. Values of MI of F1 seeds exhibited slight increasing than those of the parent treatments of both synthetic and bio-fungicides. On the other hand, the MI of F1 plants of the Rizolex (synthetic fungicide) were significantly lower than that of the control and it was the lowest one at all. It was clearly observed that the treated plants exhibited significant total percentage of chromosomal aberrations except treatments of 6h at the lower two concentrations of both Dithane and Rizolex. The treatment of Dithane at 12h with the three concentrations induced a highest percentage value of chromosomal aberrations (19.7%and 19.8%) when compared with control plants. There was no significant increase in the percentages of total chromosomal abnormalities among the two bio-fungicides (Blight Stop and Clean Root) and that of the control. The values of total abnormal cells of F1 and F2 exhibited highly decrease than those of the parent treatments (both synthetic and bio-fungicides). There were no significant differences between treated and control plants in chiasma frequency/cell for all used fungicides. The treated parent plants with synthetic fungicides (Diathane and Rizolex) have a significant proportion of abnormal pollen mother cells (10.01% and 10.06%, respectively) than those of control and treated plants with bio-fungicides and there was a significant difference of the total abnormal (PMCs) in F1 plants between synthetic fungicides Dithane and Rizolex (8.68% and 10.304% respectively) compared with control (3.54%), On contrast, there was no significant effects in the percentage of total abnormal (PMCs) between bio-fungicides Blight Stop and Clean Root (5.494% and 2.587% respectively) and control plants.  It could be concluded that from the cytogentical point of view the use of bio-fungicides as an alternative agricultural material in spite of the synthetic pesticides may become very safe.