The present investigation was conducted during two seasons (2007 and 2008) on Japanese persimmon trees located at commercial orchard, cv Costata, which considered the main and early ripening cultivar. Spraying trees with ascorbic acid (AA) and cyanocobalamine (B₁₂) in single or combined treatments were applied at different stages of development. Generally, fruit firmness values were gradually reduced from zero time of storage till the end of storage period in all treatments of AA and vitamin B₁₂ alone or in combination. The influence of both AA and B₁₂ in maintaining fruit firmness at harvest time and during the storage studied or shelf-life' resulted in increasing fruits firmness than the control. Total Soluble Sugars increased by applying AA and B₁₂ sprays alone or in combination at different concentrations. Total tannins content of fruits decreased significantly during shelf-life period. Generally all treatments of AA and B₁₂ alone or in combinations decreased total tannins content. Total phenolic contents significantly increased in the persimmon fruit as affected by different treatments during per-harvest, period, whereas AA alone at 0.5 and 1% have achieved the highest contents of total phenolic substances followed by AA combined with B₁₂ sprays at two season of the study at harvest time. Ion leakage percentage increased with advancing the shelf-life period till the end of storage to 15 days, as affected by different treatments applied of AA and B₁₂ alone or in combination. It is apparent that tree sprayed with AA and B₁₂ has less ion leakage than control fruits during shelf-life period.