This study was carried out at Bany El-Hareth site north of Sana'a- Yemen to evaluated the ability of Brassica juncea (L.), which has already been recognized as a plant suitable for metal phytoremediation to extract Lead (Pb), Cadmium (Cd), Nickel (Ni), Zinc (Zn), and Copper (Cu) from waste water contaminated soil and investigated the effect of plant parts and contaminated soil on heavy metals accumulation in plant. Indian mustard plant ( B. juncea ) was grown in wastewater contaminated soil. This study showed that Brassica juncea (L.) was able to grow in heavy metals contaminated soils and also able to accumulate extraordinarily high concentrations of some metals such as Pb, Cd, Ni,  Zn and Cu in its tissues. The results indicated that contaminated soil content of Pb, Cd, Ni, Zn and Cu decreased 45, 56, 73, 53and 79 % respectively after plant harvest. Plant parts and contaminated soil affected heavy metals accumulation in plant, the highest accumulation 54.0, 24.8 and 42.3 ppm of Pb, Cd and Ni respectively were in plant roots, whereas, the highest accumulation of Zn was 250.6 ppm in shoots and 35.6 ppm of Cu in fruits.