This experiment was carried out during 2013- 2014 (first ratoon) and 2014-2015 (second ratoon) seasons to study the effect of spraying seaweed extract at three concentration 0, 0.1, 0.2 % and/or trimming (without one & two hand) on Grand Nain banana bunches in sandy soil. Trimming of hands was done after bunch shooting, seaweed extract sprayed three times: 1) after bunch shooting directly, 2) 15 days later 3) 30 days later. Time to harvesting, bunch weight (kg), estimated yield (ton), estimated increasing rate of yield (%), finger diameter (cm), finger weight (g) finger length (cm), soluble solids content (SSC) and titratable acidity percentage were determined. Obtained results showed that trimming with two hands gave the highest values for the above mentioned characters compared with one hand. Time of harvesting tended to decrease with hand trimming of banana. The highest values of bunch weight, yield, increasing rate of yield and finger parameters were obtained from Grand Nain banana plants trimming two hands with spraying seaweed extract at 2 % .Seaweed (Ascophyllu mnodosum L.) is a known source of plant growth regulators which an important roles in metabolism and productivity of plants. Moreover, hand trimming is practiced to increase the finger parameters. From the obtained results it could be concluded that, spraying seaweed extract at 2% with trimming two hands improved time to harvesting, yield as well as physical and chemical characteristics of the fruits of Grand Nain banana plants. A superior effect was attributed to using seaweed extract and trimming rather than control.