Two field experiments of rocket (Eruca sativa Mill) were conducted during the two seasons of 2015 and 2016 at the Experimental Station Farm of the Faculty of Agriculture, Alexandria University, at Abeis, Alex. Governorate, A.R.E, to study the effect of autoclaved cellular content of Spirulina platensis as a foliar spray, comparing to N/P/K chemical fertilizer, on vegetative growth, yield, antioxidant, mineral contents and the cytotoxic effect of rocket cultured after harvested against the human cancer cells A549, and HepG2. Seeds of rocket were sown on 25^th of March, in both seasons. Four treatments were investigated in this experiment; three treatments from extract of S. platensis (5%, 10%, and 15%) and the fourth one was NPK fertilization as a control treatment. The results indicated that the highest significant plant height was observed with the foliar spraying of S. platensis at the rate of 15 % (43.33 and 43.63 cm, respectively). However, S. platensis (5 %) exhibited the lowest significant plant height (35.00 and 36.66 cm, respectively). The highest significant number of leaves was recorded with S. platensis at the rate of 5 % (8.33 and 9.00, respectively). On the other hand, N-P-K control treatment showed the lowest significant number of leaves (6.67 and 7.00, respectively). Also, the results indicated that treatment of S. platensis 10 % was responsible for  the highest significant total yield, in both seasons (2.32 and 2.29 kg/m², respectively), followed by S. platensis 5 % (2.03 and 2.08 kg/m², respectively).On the other side, the treatment of S. platensis 5% exhibited the highest positive significant antioxidant activities of all five assays, except for DPPH. In general, under the conditions of this study, it could be concluded that, the foliar spraying of 5% conc. S. platensis three times (10, 18 and 26 days after sowing), achieved the best significant quantitative (vegetative growth and yield) and qualitative (total antioxidant activity) characteristics of rocket plants. Interestingly, this concentration achieved anticancer activity (61.3 %) against hepatocellular carcinoma cell line (HepG2).  In addition, this treatment is very safe for human, decreased the applied NPK-dose by 100 %. This in turn would reduce the costs, environmental pollution and improving the human health.