Two field experiments were conducted through summer seasons of 2016 and 2017 to evaluate application of a mixed inoculation (In)biofertilizer (Bradyrhizobiumsp., B. megatherium and B. circulana) and/or moringa leaf extract (MLE) on yield and parameters of cowpea plant. The following parameters were determined: plant height (cm), number of branches/plant, number of leaves/plant, leaf area, fresh weight, dry weight of plant, peduncle number/plant, number of total pods/plant, weight of pods/plant, seeds yield, seeds yield (kg/fed.) and weight of 100 seeds. Also, the percentages of protein, ash, fats and fibers were estimated. The results generally, showed no significant increases for those characteristics due to mixture biological inoculation, although, the mineral fertilizers used with inoculated treatments were very low compared to not inoculated-chemical fertilized control (100% NPK), inoculation treatments were received 25% N, 50% P and 50% K. While the cowpea seeds treatment with moringa leaf extract before planting induced significant increases in these traits over control. Moreover, the application of moringa leaf extract as seeds treatment and foliar spraying were significantly increased these characteristics over not treated (control) and seeds treatments too. The treatment of inoculation with seed treatment and spaying with moringa leaf extract at 25% N, 50% PK [In (25% N + 50% PK) + S + M] was the best in this context which attained the highest records in all studied yield components especially seed yield (1263.8 kg/fed.) that represent the highest seed yield. The economic analysis for data of this experiment revealed that this treatment was also the most economic one, whereas exhibited the highest net return (8938.52L.E./fed.) compared to (6309 L.E./fed.) for control treatment (100% NPK), with a difference about (3130 L.E./fed.).