The widely buffalo's milk and its products consumption in Egypt, in additional to  high nutritional values of buffalo's milk make these products target for potential adulteration with different types of milk, especially cow's milk. In the present study, 100 samples of buffalo's milk and its products were randomly collected and investigated for the presence of cow's milk by using SYBER green-based real-time PCR. Samples were collected from local market in Mansoura city, Egypt. Raw buffalo's milk, yogurt, fresh Domiati cheese and pickled Domiati cheese samples were found to contain cow's milk, the presence rate of cow's milk in raw buffalo's milk more than yogurt and Domiati cheese. To determine the sensitivity limit of SYBER green- based real-time PCR using experimental samples of buffalo's milk, buffalo's yogurt and buffalo's Domiati cheese, including different concentrations of cow's milk (0.5, 1, 2, 5, 10, 20, 30 and 40%) which revealed the efficiency of method detection limit till reached 0.5% of cow's milk in buffalo's milk and its products. However the detection limit of cow's milk in buffalo's yogurt and cheese were 1 and 2% respectively.