The present study was carried out to elucidate the molecular characterization of six peaches (Prunus persica L.) strains derived from Egyptian Meet-Ghumr cultivar. In addition, further understanding of the structure of genetic diversity using sixteen previously described SSR primer pairs to determine if the primers could discriminate between the strains. The loci were low polymorphic, the different alleles found at each locus only ranged from 1- 4 including null allele. The observed heterozygosity ranged from 0.00 - 0.66, the heterozygosity level within the genotype varied from 0.00 - 0.30, gene diversity or the polymorphic information content (PIC) values were between 0.00 to 0.51, and the power of discrimination (DP) for each locus differed from 0.00 – 0.61. Regarding the probability of matching fingerprints, two of the strains 'Soltany and Shamiea' were identical in the DNA profile with all the sixteen loci and thus considered to be genetically identical. The similarity degree ranged from 0.83 to 1.00 with an average of 0.91, the lowest similarity degree was between Mawwy and Farkk Meet-Ghumr, indicating the low level of genetic variation that exists in the edible peach gene pool. This is confirmed the importance of benefiting from other genetically close species of peach such as almond in the genetic improvement of peach.