Background: The vascular catheters uses is common in both inpatient and outpatient care that play an integral role in modern health care and are of great clinical value for supporting patient's status. Nursing management of peripheral vascular lines is important. A comprehensive understanding of the procedure features an excellent value so as to scale back the complications arising from peripheral vascular lines. All staff must remember all aspect of care and therefore the principles of safe insertion, injection, dressing change & flushing procedure. This study aimed to evaluate the effect of an educational program on nurses' knowledge regarding management of patients undergoing peripheral vascular access. Subjects and method: Design: quasi-experimental design (pre and posttest design) was utilized to conduct the study. Setting: study was conducted at Al-Azhar university hospital in New Damietta city. Subjects: The study nurses consisted of 50 nurses. Tools: self- administered questionnaire sheet to assess nurses' knowledge regarding management of patients undergoing peripheral vascular access. The Results: Majority of nurses (94%) had satisfactory total knowledge immediately post educational program implementation compared to (18%) pre program and (86%) of them follow up after educational program implementation. Conclusion: There was statistically significant difference in nurses' knowledge between pre and immediately post program implementation Recommendations: Providing ongoing education and practical training for areas of low performance to make sure the standard of care provided by nurses.