Background: The vascular catheters uses is common in both inpatient and outpatient care that play an integral role in modern health care and are of great clinical value for supporting patient's status. Nursing management of peripheral vascular lines is important. A comprehensive understanding of the procedure features an excellent value so as to scale back the complications arising from peripheral vascular lines. All staff must remember all aspect of care and therefore the principles of safe insertion, injection, dressing change & flushing procedure. Aim of the study: To evaluate the effect of an educational program on nurses' practice regarding management of patients undergoing peripheral vascular access. Research design: Quasi-experimental design was utilized to conduct the study at Al-Azhar university hospital in New Damietta city. The study nurses consisted of 50 nurses. Tools: Two tools were used for data collections: tool I Structured interview questionnaire sheet and tool II Nurses' practice Observational Checklists. The Results less than three quadrant of studied nurses (70%) had satisfactory total practice level immediately post educational program implementation compared to (12%) of studied nurses pre educational program implementation and  (22%) of subjects follow up educational program Conclusion: There was significant improvement in nurses' practice between pre and immediately post program implementation Recommendations: Providing ongoing education and practical training for areas of low practice to make sure the standard of care provided by nurses.