Background: Upper gastrointestinal bleeding is a potentially life-threatening abdominal emergency that remains a common cause of hospitalization. The role of the nurse in managing a patient with upper GI bleeding requires specific attention. Aim: Evaluate effect of implementing training program regarding care of patients with upper GIT bleeding on nurses' knowledge. Subjects & Method: Design: A Quasi experimental (pre/post –test) research design was used: Setting: study was conducted at Hematemesis Unit of Emergency Hospital located in Critical Situations and Convalescence Hospital affiliated to Mansoura University Hospitals.  Subjects: Convenient samples of 25 male and female nurses who are dealing with upper gastrointestinal bleeding patients are selected from Hematemesis Unit of Emergency Hospital, Mansoura University, Egypt. Tools: Two tools were used to collect data; demographic characteristics of the nurses and nurses' knowledge assessment self-administrated questionnaire .A Quazi experimental (pre/post-test) design was used. Results: of the present study revealed that there was a highly statistically significant improvement in total knowledge of nurses regarding care of patients with upper gastrointestinal bleeding with p-value of (<0.001). Conclusion: the training program has a remarkable significant improvement on nurse's knowledge. Recommendations: a continuing training program be planned for and offered on regular basis to nurses in Hematemesis Unit to improve their knowledge regarding care of upper gastrointestinal bleeding patients and periodic monitoring of nurses knowledge by nursing audits and supervisors.