Background: Once Corona virus disease 2019 discovered in December 2019 at Wuhan, China, is considered an emerging respiratory disease developed by a novel coronavirus. Effect on 10%-20% of total healthcare workers, mainly were nurses due to direct contact with the cases. Aim: The aim of this study was to assess nurses' knowledge, practice, attitude, and psychological stressors regarding COVID-19 at quarantine hospital. Methods: A descriptive cross-sectional research design was used. Subjects: A Convenient sample of 183 nurses participated in this study. Tools: Utilize the study with two online tools; Tool I, self-administrated questionnaire to assess level of knowledge, practice, and attitude. Tool II, COVID-19 psychological stressors questionnaire to assess the varied COVID-19 stressors among the studied nurses. Results: The findings revealed that the studied nurses had a satisfactory level of knowledge and practice. Majority of them had positive attitude (98%) and null stressors (48%) toward COVID-19. In addition, there is a significant correlation between nurses' knowledge level and COVID-19 psychological stressors. Conclusion: The study concluded that the studied nurses were satisfied and had a positive attitude with null stressors regarding COVID-19. Recommendations: Continuous updating nurses' knowledge and practice. Ensure all facilities to promote their attitude, psychological support to battle the present pandemic.