Background:Handle and administer of antineoplastic chemotherapy can cause negative health effect among nurses prepare, and administer antineoplastic chemotherapy. Aims of the study:explore the nurse'sperformance and adverse health effects on nurses dealing with chemotherapeutic Agents. Design:A correlation descriptive research design. Setting: This study was conducted at three chemotherapeutic units in oncology Institution in Damietta, Port Said general hospital and EL Tadamon hospital. Sample: A Purposive sample of 48 nurses, oncology Institution in Damietta (N=41), Port-said hospitals (N=7) in period of 6 months. Data collection: Two tools were used for data collection: A Structured self-administered questionnaire and Nurses' performanceobservational checklist. Results:Only 57.9% of the studied nurses had unsatisfactory level of general knowledge, while 52.3% of them had satisfactory level of practice, with regards to the adverse health effect the results shown that 54.8%, 51.6%, 41.9%, 38.7% and 35.5% s inflammation of the skin, fatigue, loss of weight, ulcers in the mouth and hair loss, headache, loss of appetite, change in nail color and changes in menstrual cycle respectively. Conclusion: There are statically significant relation between nurses' level of performance and the occupational health hazards. Recommendations:periodiceducational and training programs to improve nurses'performance regarding use of chemotherapy and protection from its health hazardsare needed.