Background: Implementing an educational program by pediatric nurse for hygiene activities, prevention and control of pandemic influenza transmission in schools is very important especially when there is a major public health threat like influenza pandemic.      Aim:  This study was aiming to assess student's knowledge regarding prevention and control of pandemic influenza transmission. It also aims to design, implement and evaluate an educational program for students' about prevention and control of pandemic influenza transmission.      Materials and Methods: A quasi-experimental design was used. Setting: The study was conducted in four governmental preparatory and secondary schools in Ismailia. Subjects: The study involved 204 students from both gender and in the age group of 12-18 years. Tool of data collection: Data were collected using A self-Administered Questionnaire   Results: Total knowledge score regarding prevention and control of pandemic influenza transmission were improved, as good score was 0% pre program while it reached 85.3% after implementation of the educational program and 84.3% after one month later.  Conclusion: Implementation of educational program for students about prevention and control of pandemic influenza significantly improved the students' total level of knowledge.  Recommendation: The study recommended educational programs about prevention and control of pandemic influenza transmission for students according to their actual need assessment.