Background: Hepatitis C virus infection is the major etiological agent of chronic hepatitis and liver disease worldwide. It is estimated that approximately 130-210 million individuals of the world population are chronically infected with HCV. Egypt has the highest prevalence of HCV worldwide, representing 15% of the general population in Egypt.  HCV is responsible for almost 90% of infections that cause chronic liver disease and death in the country. Objective.: The aim of this study was to evaluate the effect of an educational program on chronic viral hepatitis C patients' knowledge related to Hepatitis C Virus disease treated by pegylated alpha interferon and ribavirin combination therapy.Subject and methods:the  research design: was quasi experimental. The sample constituted of 216 hepatic C patients was classified into two equal groups: control and study, treated by alpha pegylated interferon and ribavirin combination therapy in El-Tadamon hospital in Port -Said city. The tool that is used for data collection was hepatitis C virus patient's knowledge structured interview sheet. The results: revealed that there was an improvement in the level of knowledge in the study group rather than the control group with a statistically significant difference,  immediately, and after three and six months of the program.conclusion: The study concluded that implementation of the designed health education program was successful in improving patients' knowledge.Recommendations: The study recommended development of a health education unit in each specialized liver center with the availability of qualified and trained nurses. Nurses should hold sessions to provide health education and counseling for these patients.