Background: Now adays wound infection is one of the most challenging aspects of wound management. It is a major contributor to healthcare costs around the world, and causes significant distress to patients and careers.  Aim: to evaluate the effectof training program on nurses' performance regarding infection control measures in caring for patient with post operative wound. Setting: The study was conducted in surgical units and out-patient surgical clinics of three general hospitals in Port-Said City. Sampling: The study was applied on 40 nurses working in the previously mentioned setting.  Data was collected through a "questionnaire sheet" to assess nurses' knowledge and "observational checklists" to assess nurses' practice in caring for patients with post-operative wounds.   Results: the studyresulted that, there were statistically significant relations between total knowledge of nurses' pre, post 1, and post 2, and between nurses' practice pre, post 1 and post 2. The results showed marked improvement in nurses' knowledge and skills related to the care of wounds after surgery after the program. Conclusion: The study concluded that there was statistically significant relation between nurses' total score of knowledge and total score of practice related to post-operative wound care. Recommendations: The study recommended that further programs with continues follow up for nurses in all general hospitals in Port-Said City to help them to improve their  performance regarding postoperative wound care through applying infection control measures and monitoring periodically the nurses' performance.