Background: Coma is a state of prolonged unconsciousness in which the patient cannot be aroused even with painful stimuli. Coma in children is categorized into traumatic and non- traumatic (NTC). Aim of the study: was to assess nurses' knowledge and practice regarding care of comatose children at pediatric intensive care units. Subjects& Method: Research design: A descriptive design was used. Setting: The study was conducted at Pediatric intensive care unit in Pediatric hospital at Zagazig University Hospitals, Pediatric intensive care unit at Al Ahrar Hospital and Pediatric intensive care unit at General Hospital. Subjects: The study sample included all accessible nurses who work at the previous setting. Tools of data collection: Two tools were used to collect the necessary data. The first was a structured interview questionnaire. The second was observational checklist for nurses' practice. Results: The study results revealed that the total scores of nurses' knowledge were good, fair and poor with percentage 69.2, % 27.7% and 3.1% respectively while the total practices level of all studied nurses were unsatisfactory. Conclusion: The study concluded that more than two third of studied nurses had good knowledge regarding care of comatose children while all studied nurses' practice provided to comatose children was unsatisfactory. Therefore, the expected care provided to comatose children was unsatisfactory. Recommendations: The study recommended that continuous formal training programs about care of comatose children will help nurses to update their knowledge and improve their practice was recommended.