The aim of this study was to evaluate the differences in the level of Physical fitness between Assiut city (Upper Egypt) and Mansoura city (Lower Egypt) among primary school age children from 7-11 years old. Participants were primary school aged-children (n = 474 Assiut city and 237 from Mansoura city ranging in age from 7 to 11 years old. The data were collected by using the German motor test. No significant differences were found between cities in the investigated participants' anthropometric parameters. A significant difference between the performances of participants from Assiut and those from Mansoura were found for the majorities of tested parameters (except LJ) with children from Assiut perform better in Sprints and BB, and children from Mansoura city perform better in strength tests such as JS, PU, SU and in 6min endurance test. These results demonstrated the effect of environment on the physical fitness levels during childhood.