Abstract T he current study aimed at developing chemistry teacher preparation program at Faculties of Education in light of Nanotechnology concepts and applications. To achieve such an aim achecklist of Nanotechnology concepts and applications ought to be incorporated in chemistry teacher preperation program in Faculities of Education, Two other standdard checklests ought to be considered in the objectives and content of chemistry teacher preparation program in faculties of Education in light of Nanotechnology concepts and applications were also prepared. Then, the objectives and content in chemistry teacher preparation programs at Banha, Ain shams and Alexandria universities were analyzed in light of the final version checklist of objectives and content standards. Based on the results of evaluating chemistry teacher preparation program in faculties of Education, aproposed ideation of chemistry teacher preparation program concepts and applications was prepared. The results revealed that the level of objectives and content of chemistry teacher praparation program at Banha, Ain shams and Alexandria universities concerning the objectivies and content standards in light of Nanotechnology and its applications is weak / poor.