Mice's reproductive system, endocrine glands, and central nervous system are all toxic and carcinogenic when exposed to acrylamide (AC). The goal of this study is to show how Nano-curcumin differs from Curcumin in minimizing the effects of acrylamide in male mice. The mice were divided into five groups: control, control (Tween-80), Acrylamide (AC) group, AC+ Curcumin (Cur), and AC +Nano-curcumin (N.Cur). An increase in Caspase-3 in the testis was induced in the AC treated group. AC decreased albumin, IgG, and antitrypsin while increasing blood protein fractions like plasma transferrin. In addition, AC increases the amount of total white blood cells and neutrophils in the CBC while decreasing lymphocytes, RBC, and hemoglobin levels. The effects of N.Cur and Cur were reversed, and several severe histopathological alterations. N.Cur and Cur also reduced the amount of collagen fiber and the percentage of fibrosis in male mice generated by AC and corrected the AC-induced glycogen depletion. Finally, the efficiency of Nano curcumin can reduce the detrimental effects of acrylamide in the testis of mice.