Morphological and anatomical characters of stem, petiole and leaves of 16 selected medicinal taxa of family Lamiaceae collected from Bani Matar district, Sana'a (Yemen) were examined using both stereo-and light microscopes. The obtained macro-and micromorphological characters (298) were analysed using the software jmp version 13. The resulted dendrogram was discussed on the light of the current taxonomic treatments of Lamiaceae. The measurement of the quantitative characters of macro-and micromorphological characters for the studied taxa was established by multivariate analysis of variance (ANOVA). Evaluation of obtained results revealed that, many characters viz. leaf composition, stomatal types and distribution, number of stamens, stem and petiole vasculature, types of trichomes, nutlet features, number of palisade and spongy layers and types of crystals are of diagnostic value in species identification and delimitation and are potentially valuable at different taxonomic levels. All the quantitative macro- and micromorphological characters except nutlet length are highly significant in species delimitation.