Background: Trophic feeding is the practice of feeding minute volume of enteral feeds in order to stimulate the development of the immature gastrointestinal tract of the preterm infants with no evidence of any adverse effects following trophic feeding. Aim of the study: This study aimed to assess nurses' performance regarding trophic feeding for preterm infants at Neonatal Intensive Care Units. Design: A descriptive design was used to conduct this study. Setting: This study was conducted at Neonatal Intensive Care Unit of El-Fayoum General Hospital. A convenient sample composed of 65 nurses who were working at the previously mentioned setting. Tools: three tools were used in this study are, pre-designed interview questionnaire sheet, observational checklists and attitude Likert type rating scale. Results: It was found that, more than two fifth of the studied nurses had a poor level of knowledge and more than two thirds of them had incompetent level of practices and more than half had positive attitude regarding trophic feeding. Conclusion: There were highly statistically significant differences with positive correlations between knowledge, total practices and total attitude of the studied nurses. Recommendations: Setting educational and training programs for neonatal nurses to improve their knowledge and practices regarding trophic feeding at NICUs.