Background: Ovarian hyperstimulation syndrome (OHSS) is a common and severe
complication for patients who undergoing invitro fertilization (IVF). Aim: To evaluate the
effect of Instructional guidelines on invitro fertilization nurses' knowledge and preventive
measures regarding ovarian hyper stimulation syndrome. Design: Quasi experimental
research design was used in this study. Setting: It was conducted at Amshaj IVF private
center & Ajyal IVF private center in Sohag city. Sample: purposive sample of all nurses
working in previous mentioned centers (30). Tools: Two tools were used in this study (1)
Structured interviewing questionnaire, (2) Observational Checklist for IVF Nurses' practice
Regarding Preventive Measures of OHSS during IVF cycle. Results: There is highly
statistical significance difference between pre & posttest regarding total knowledge level and
total practice level of preventive measures about OHSS of the studied nurses . Conclusion:
Implementation of the instructional guidelines was effective in improving IVF nurses'
knowledge level and practice of preventive measures regarding OHSS. Recommendations:
Implementing this instructional guidelines with a learning booklet for nurses on the other
IVF centers to improve & update their level
of knowledge and practice of preventive
measures regarding ovarian hyperstimulation Syndrome.