The efficiency of public health initiatives focused on eradicating infectious diseases has been
seriously threatened by the COVID-19 vaccination. The current study aimed to evaluate the efficacy
of educational intervention regarding COVID-19 vaccination on nurses' knowledge, attitude, and
acceptance. The study was conducted at medical outpatient clinics affiliated with Mansoura
University Students Hospital, Mansoura, Egypt. A quasi-experimental research design was used
(pre-post educational intervention). A convenient sample of 150 nurses was included in the study.
The study was collected by three tools to assess demographic and occupational characteristics of
nurses, medical health history and their knowledge, attitude and acceptance. The study's findings
revealed that 82.7% of the studied nurses had a satisfactory total knowledge score level in the post
test compared to (4.7%) in the pre-test. The differences observed were highly statistically significant
at (p value<0.001). Moreover, the total mean score of the studied nurses' acceptance significantly
improved from 3.35 ± 1.73 to 6.09 ± 1.36 pre and post at a p-value <0. 001. Conclusion: The
application of the educational intervention showed a significant improvement in nurses' levels of
knowledge, attitude, and acceptance regarding Covid-19 Vaccination. Well-structured training
programs targeting all nurses in Egypt must be launched to improve their knowledge and attitude
toward Covid-19 Vaccination.