Background: Using OSCE can help educators to evaluate students' competency, based on objective testing as well as it is foster students to develop many skills as problem solving, decision making, emotional intelligence, feedback seeking behaviors, emotional stability, and clinical judgment. The aim of the current study was to investigate the effect of OSCE on nursing students' emotional intelligence and feedback seeking behavior. Research design was a descriptive correlational design. Setting was Faculty of Nursing at Minia University. Subjects: included all first year and fourth year nursing students in the academic year 2018-2019 (N =717). Tools: Three tools were utilized to collect pertinent data for this current study. Results: the fourth year students had high score for OSCE feedback, EI, FSB than the first years who had moderate score. Conclusion: the current study concluded that the fourth year students had high score regarding their OSCE feedback, EI, and FSB than the first year. Also, there were positive correlations between the using of OSCE and students high level of EI as well as high level of FSB. Recommendation: encourage the use of OSCE to improve students' ability clinically, emotionally and cognitively.