Background: Most postpartum complications are considered preventable if postpartum observation and examination is conducted effectively. Around 216 women died worldwide from 100,000 women due to postpartum complications. Thus, Aim of this research was to investigate effect of Moodle based learning on maternity nursing students' knowledge and skills regarding fundal assessment during puerperium. A Quasi- experimental research design was used. A simple random sample of 80 maternity nursing students was randomly divided into two equal group, namely; Moodle group and traditional group. The study was conducted at Obstetric and Gynecological Nursing clinical training laboratory and information technology laboratory at the Faculty of Nursing, Alexandria University and El-Shatby Maternity University Hospital. Tools: three tools were used: (1) Maternity nursing students' knowledge regarding fundal assessment during puerperium questionnaire, (2) Maternity nursing students' skills regarding fundal assessment during puerperium observational checklist and (3) Maternity nursing students' satisfaction about using Moodle based learning in performing fundal assessment during puerperium. Results: revealed a statistically significant difference between the study subjects regarding Moodle group in relation to their knowledge and skills immediately, two weeks and two months after teaching sessions. The study concluded that students using Moodle based learning had better knowledge and skills retention than those of the traditional group at all three time periods.  It was recommended that replication of the same study in different Egyptian faculties of nursing to introduce the concept of Moodle based learning and explore its importance for undergraduate and postgraduate nursing students.