Arterial blood gases analysis considered a method for monitoring high-risk neonate's condition as it
provides the basic information for determining the adequacy of alveolar ventilation, acid-base status and
oxygenation. Nurses' attention should be given to the following factors; infection control or universal
precautions as the neonates are at high risk of infection. Aim: The study aimed to evaluate the effect of a
computer-based learning module on nurses' performance regarding safety arterial blood gases sampling
for high risk neonates. Methods: A quasi-experimental research design conducted at Neonatal Intensive
Care Units (NICUs) and Surgical Neonatal Intensive Care Unit "SNICU" of Benha Specialized Pediatric
Hospital. A purposive sample of available nurses (62) and high-risk neonates (74). Researchers used
three tools as Online nurses' self-administered questionnaire designed to assess nurses' personal
characteristics and nurses' knowledge regarding safe blood sampling for high-risk neonates. The second
tool was nursing practice observation checklists. The third tool was High-risk Neonates' Medical
Assessment Record. Results: Findings of the current study revealed a statistically significant difference
pre and post computerized-based learning module implementation regarding safety arterial blood gases
for high-risk neonates. The results also revealed a highly statistically significant difference in nursing
knowledge and practices (p < 0.001). Conclusion: It can be concluded that the research hypothesis is
accepted, the implemented computerized-based learning module improved nurses' knowledge and
practices towards safety arterial blood gases sampling for high-risk neonates. The study recommended
conducting periodical online programs for nurses in NICUs with continuous regular updating of
knowledge and practice regarding safe arterial blood gases sampling for high-risk neonates.