Background: In Vitro Fertilization (IVF) is a type of assisted reproductive technology used for infertility treatment couples undergoing (IVF) suffer from various problems that affect their life. Aim: This study aimed to assess infertile couples' knowledge regarding In Vitro Fertilization Process. Research design: A descriptive research design was utilized. Setting: This study conducted at Assisted Reproductive Unit at Ain Shams Maternity University Hospital. Sample: A simple random sample was used to recruit 98 couples. Tool of data collection: An interviewing questionnaire sheet was used for data collection. Results: shows that mean age of the studied husbands is (35.97 ± 5.47) years while, the mean age of the studied wives is (29.72 ± 4.14) years. As regard infertile couples total level of knowledge regarding IVF process only (12.8571 ± 2.55767)of the wives have satisfactory level of total knowledge regarding IVF process while, (6.0408 ± 1.45715) of husbands have satisfactory level of total knowledge regarding IVF process. Conclusion: The finding of the current study indicated that less than two third of infertile couples have unsatisfactory level of knowledge regarding IVF process. Furthermore, nearly three quarters of wives have unsatisfactory knowledge regarding factors that increase the chances of IVF success, required medications, instructions followed after the procedure, and complications of IVF process. While, nearly three quarters of husbands have unsatisfactory knowledge regarding instructions followed before and during the procedure. Recommendation: Based on the finding of the current study the following recommendation is suggested developing educational programs regarding IVF process for infertile couples on IVF unit of Ain shams maternity university hospital.