Aims and objectives. To assess nursing staffs' knowledge and practice regarding the
prevention of hepatitis C virus (HCV) transmission in hemodialysis units (HDUs). Design. A
descriptive exploratory study was utilized to fulfill the aim of the study. Subjects. A convenient
sample consisted of all hemodialysis nurses (55 nurses) in three HD units at Cairo University
Hospital, Egypt. Tools. Two instruments were designed to collect data pertinent to the study: a)
Self-administered questionnaire to assess the subjects‟ level of knowledge, and b) Observational
checklist, used to assess subjects‟ level of practice. Results. Showed that (54.5%) of the studied
subjects have got a satisfactory level of knowledge regarding prevention of HCV transmission,
while all of them (100%) have got an unsatisfactory level of practice related to prevention of
HCV transmission in HD units. Recommendations. In-service training program should be
designed and implemented regularly to all hemodialysis nurses.