Background: Vaginal infections are a global health problem for women at reproductive age. These infections threat the women‟s health and also have negative impacts on their health. Aim of the study: to evaluate the effect of self-care guidelines on knowledge and practices, among faculty of nursing students with vaginal infection. Subjects and methods: quasi experimental research design was used to conduct the study at Faculty of Nursing, Ain Shams University. It included 214 female students selected by purposive sampling technique according to inclusion criteria, who suffer from vaginitis. The data were collected by using a self-administered questionnaire including socio-demographic data, knowledge, and practices assessment tool. The data was collected through a period of 8 months from October 2017 to May 2018. Results: the prevalence of vaginal infection among female students constituted almost the quarter. The majority of studied students lacked the essential knowledge regarding vaginal infection in the pretest and have unsatisfactory practices score. Meanwhile, after conduction of self care guidelines, it observed that there was a highly significant improvement in their knowledge and practice. Conclusion: There were statistical significant difference between total knowledge and total practices (P<0.001) pre and post conduction of self care guidelines. So self-care guidelines about vaginal infection were effective in improving the knowledge and practices for nursing students with vaginal infection. Recommendation: Applying self care guidelines for adolescent females and their mothers to increase awareness about vaginal infection. Develop a special health center for adolescent females motivate them to seek prevention, early diagnosis and treatment of vaginal infection that may have positive impact on their future health.