Nurses play a crucial role during provision of care, as they are responsible for maintain children safety in the hospital. Aim of the study: was to assess the effect of guidelines for nurses' performance regarding children safety in Benha hospitals. Design: A quasi-experimental design was utilized in this study. Settings: the study was conducted in pediatric departments at Benha University Hospital and Teaching Hospital. Sample: A convenient sample included 70 nurses (pre and post test). The tools of data collection: Three tools were used in this study. tool (I) a structured interviewing questionnaire to assess nurses knowledge regarding child safety, tool (II) hospital survey on child safety culture to assess nurses attitude, tool (III) an observational checklist followed by designed guidelines booklet. Results: The present study revealed that a statistical significant difference of total knowledge, attitude and practice scores of the studied nurses regarding children safety related age, years of experience and qualification during post-intervention. There was positive correlation coefficient between nurses' total knowledge, attitude and practice scores in favor of post intervention. Conclusion: The present study revealed that there was highly statistically significant difference between knowledge, practice and attitude of the studied nurses regarding children safety during pre and post intervention of guidelines regarding children safety. Recommendation: Application guidelines regarding children safety for the nurses working at all pediatric departments to improve their knowledge, attitude and practice