Background: Transition from nursing student to staff nurse can be exciting, stressful and challenging. Aim: aim of this study was to assess nurse students' perception regarding role transition at faculty of nursing - Benha University. Design: a descriptive research design was utilized to meet the aim of this study. The sample of this study included 193 out from 370 nurse students who selected randomly. Tools: tool for data collection: factors affecting role transition questionnaire. Results and Conclusion: More than two thirds of nurse students had moderate agreement level regarding organizational factors, also more than half of nurse students had moderate agreement level regarding personal and educational factors. Recommendation: Creating healthy environment is highly needed for new nurses during role transition. Conduct an orientation program for nursing students before internship year is very beneficial and prior each clinical training area to explain objectives, responsibilities and discuss nurse students' expectations.