Women with genital prolapse experience group of medical, psychological and social problems that affect their quality of life and their sexual activity. The present study aimed to evaluate effect of evidence based guide line regarding genital prolapse postoperative care on women's knowledge and self- care practices. An intervention study design (quazi experimental design) was conducted at Beni-suef general Hospital & Ain shames maternity hospital. A purposive sample of 60 women was included in the study. They were divided into two groups (study & control).Data were collected tools; structured interviewing questionnaire sheet, checklist, visual analog scale (VAS)and follow up card. In addition as a supportive material guide line was given to study group. The results of the study revealed that there was a highly statistical significant difference p (< 0.001)between the study and control group total score of knowledge and self -care practices of women at pre-intervention, post and follow up in which the study group had higher score than the control group p (< 0.001). The study concluded that evidence based guide line proved to have a positive effect on knowledge and self-care practices of women undergoing surgery for genital prolapse, so study recommended that disseminate the application of multidisciplinary collaboration approach for postoperative evidence based guide line on women with genital prolapse surgery, further researches regarding nurse's perception and practices about evidence based guideline for genital prolapsed nursing care.