Aim: This study aimed to assess the impact of an educational program on nurses' knowledge and performance regarding nursing care of children with WT. Setting: This study was conducted at the outpatient and inpatient of pediatric oncology departments in Children Cancer Hospital (57357). This study sample is convenient, involved all the available (60) staff nurses giving nursing care for about 70 children suffering from WT. Tools of data collection included a structured interviewing questionnaire and Observational checklists sheets for assessing nurses' knowledge and performance (pre-posttest and follow up). The findings of the present study revealed that, the impact of the educational program on nurses' knowledge and performance about care of children with WT the result concluded that there was a positive correlation between nurses' knowledge and performance regarding WT after the educational program implementation. The result recommended that newly appointed nurses who are working at oncology unit must have an orientation program prior to care of children with Wilms' tumor, periodical continuous on job-training programs for oncology nurses to update and refresh their knowledge and performance about care of children with Wilms' tumor, regular clinical sessions to monitor the nursing performance at 57357 hospital will reinforce and improve the skills regarding to care of children with Wilms' tumor and further studies and replication of this study in other oncology units.