Parkinson's disease (PD) is considered the second most common neurodegenerative disease with subsequent motor and behavioral deficits. Oxidative stress plays a key role in the pathogenesis of PD. AIM: The aim of this study was to investigate the possible neuroprotective effects of crocin on rotenone-induced Parkinson- like behaviors. MATERIALS &METHODS: 70 male adult Wister Albino rats were randomly divided into 4 groups: (1) Control (10 rats); (2) Crocin 40 (10 rats); (3) Polyethylene glycol (PEG) (10 rats) ; (4) Rotenone (40 rats) injected I.P. of 1.5 mg/kg/48 hrs. for 2 weeks [11]; Preliminary behavioral tests and the rats that showed PD features were randomly subdivided into 4 equal groups of 10 rats per each. (4A) Rotenone-treated: (4B): Crocin 20 treated. (4C): Crocin 40 treated. (4D) L-DOPA treated- group. The neurobehavioral were done. In serum, the level of 8-hydroxydeoxyguanosine 8-OHdG was estimated. The level of malondialdehyde (MDA), reduced glutathione (GSH), tumor necrosis factor alpha TNF-α, dopamine, and nitrite/ nitrate levels were measured in the brain tissue. RESULTS: Rotenone induced neurobehavioral deficits with elevation of brain MDA, brain TNF- α, Nitrite/nitrate level and serum 8-OHdG and reduction of GSH, brain tissue dopamine. Crocin (20 or 40) improved these neurobehavioral deficits. Crocin (20 or 40) and L-DOPA decreased MDA, serum 8-OHdG, TNF- α and Nitrite/nitrate level and increased GSH and dopamine level. Crocin 40 had achieved potent effect compared with crocin 20. In summary, rotenone-induced Parkinson- like behavior in rats. Crocin achieved a protective effect