Chitosan (CS) as an inexpensive bio-adsorbent was set by chitin deacetylation using sodium hydroxide. The prepared bio-adsorbent was distinguished via Scanning electron microscopy (SEM), X-ray diffraction (XRD), Energy dispersive spectroscopy (EDX), Brunner-Emmett-Teller surface area (BET) analyzer, and Fourier transform infrared spectroscopy (FTIR). Chitosan implemented for U(VI) adsorption, and the studied parameters that are pH, time of contact, initial concentration, and temperature, were determined by batch technique. The maximum uptake of CS adsorbent is 88.0 mg/g at 200 mg/L initial U(VI) concentration, pH 3.5, and 25 °C.  The kinetic information agreed with the pseudo-second-order model, which considered the attendance of chemisorption. The equilibrium of adsorption reaction gained within 50 min, and equilibrium information well satisfaction for Langmuir model confirmed that U(VI) adsorbed at chitosan monolayer coverage area. Also, the chitosan bio-adsorbent was indeed regenerated by 1M H₂SO₄ and 1/50 S/L ratio for 75 min of contact.  Seven rounds of adsorption-desorption investigations attended to scrutinize the working applicability and renewed application of the bio-adsorbent.