Toltrazuril is a veterinary antiprotozoal drug. In this work, four different simple, accurate, and sensitive spectrophotometric methods have been developed to determine toltrazuril in the presence of its alkali-induced degradation product. Two of these methods depend on selection of two wavelengths at zero order spectrum these methods include (A) dual wavelength method at 239.60 and 257.40 nm, (B) simultaneous equation method at 242.4 nm (λmax of toltrazuril) and 249.8 nm (λmax of toltrazuril degradation product),while the another two methods are (C) area under the curve  method at (235-245)nm and ( 247-257) nm of  toltrazuril and its degradation product and (D) ratio derivative method the absorption spectra of toltrazuril were divided by a suitable absorption spectrum of its degradation product (divisor) and the amplitudes of the first derivative of the ratio spectra at 219 nm were recorded. These methods have been validated for linearity, accuracy and precision and found to be rapid, precise to applied for determination of toltrazuril in Yoserzoril^® suspension.